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PeptidesEvidence: Standards-based

TB-500 Dosage Calculator: What the Math Can and Cannot Tell You

Written by
Daniel Reyes, PhD
Medically reviewed by
Dr. Sarah Lindqvist, MD
Published
June 20, 2025
Updated
June 20, 2025
Last medical review
June 20, 2025
Reviewer scope
Metabolic health
Precision pipette and test tubes symbolizing the fixed arithmetic inside every honest TB-500 dosage calculator

Any honest TB-500 calculator does exactly one job: given a vial size, diluent volume, and a dose supplied by a licensed clinician, it computes concentration and draw volume. It cannot tell you what dose to take, because no human trial has established one — TB-500 has no published human dosing data at all. Calculators that output "recommended doses" are laundering forum numbers through an interface.

The only legitimate calculator math

Concentration = vial mg ÷ diluent mL. Draw volume = prescribed dose ÷ concentration. Units on a U-100 syringe = draw mL × 100. A 5 mg vial in 2.5 mL is 2 mg/mL; a clinician-prescribed 2 mg dose would be 1 mL. That is the entire science of peptide calculators.

Preparing a syringe from a reconstituted vial — the only step where TB-500 calculator math legitimately applies

The "loading phase" numbers you will see elsewhere

Community protocols describing weekly loading then maintenance derive from veterinary practice and forum iteration — not from human pharmacokinetics, which have never been published for this fragment. We report that they exist; we do not repeat the numbers as guidance.

The compound behind the calculator has its own evidence file — the TB-500 guide. The general math is worked through in how to reconstitute peptides and the design philosophy in peptide calculators: the formula inside. Run prescribed numbers through our reconstitution calculator.

Use ours, with its limits stated

Our reconstitution calculator publishes its formula, validates input ranges, and refuses to suggest doses by design. This page reports what published studies and regulatory documents describe. It never recommends a dose — dosing is a clinical decision for a licensed prescriber who knows your history.

Frequently asked questions

How do I calculate a TB-500 dose?
You cannot calculate a dose — no human trial has established one for the TB-500 fragment. A calculator can only convert a dose a licensed clinician has already prescribed into a draw volume: concentration = vial mg ÷ diluent mL; draw = prescribed dose ÷ concentration.
What is the draw volume for a 5 mg vial in 2.5 mL?
That reconstitution yields 2 mg/mL. A clinician-prescribed 2 mg dose would be 1 mL — 100 units on a U-100 insulin syringe. The arithmetic is fixed; only the prescription varies.
Why do some TB-500 calculators recommend doses?
Those tools are laundering forum numbers through an interface. The community "loading phase" figures derive from veterinary practice and forum iteration, not human pharmacokinetics — which have never been published for this fragment.

Questions to ask a licensed clinician

  • If this were ever prescribed, what dose and schedule would you set and why?

References

  1. Goldstein AL, Hannappel E, Sosne G, Kleinman HK (2012). Thymosin beta-4: a multi-functional regenerative peptide. Expert Opinion on Biological Therapy. SourceReview
  2. US Pharmacopeia (2023). General Chapter <797> Pharmaceutical Compounding — Sterile Preparations. USP-NF. SourceStandard

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